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Image Search Results
Journal: Journal of the American Chemical Society
Article Title: Biosynthesis of 14-membered cyclopeptide alkaloids via non-heme-iron- and 2-oxoglutarate-dependent oxidative decarboxylation
doi: 10.1021/jacs.5c23400
Figure Lengend Snippet: ( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - 2-oxoglutarate, EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).
Article Snippet: Assays were 50 μL total volume with final concentrations of 50 mM HEPES pH 7.5, 200 μM FeSO 4 -7H 2 O, 500 μM
Techniques: Transgenic Assay, Expressing, In Vitro, Activity Assay